Biotechnology - Philadelphia, Pennsylvania, United States
Enzium's EnSens® technology is a new fluorescent protease activity assay technology that doesn't rely on FRET, fluorescent proteins, or immunofluorescence, and can potentially be used in any fluorescent application, from standard plate reader fluorometry to flow cytometry and confocal microscopy. In addition, EnSens® substrates can incorporate full recognition site sequence and tertiary structure, greatly enhancing selectivity and yielding more relevant, high-quality hits.\\EnSens® unique fluorescent protease assay technology offers critical advantages:\• Selective: Substrate module can accommodate up to 200aa protease recognition sequences\• Versatile: Modular, Lego™-like, protein-based substrates enable us to easily customize to your favorite protease\• Sensitive and Quantitative at picomolar levels of active protease. Repeatable, Reproducible, and Robust\• Fewer False Positives and Negatives: 6-30X signal-to-noise ratios; red wavelengths give greater sensitivity and accuracy\• Cell Based Assays: Stable, protein-based (scFv) substrate & nontoxic dye allow cell surface detection of protease activity\• Bead Based Assays allow for multiplexing in flow cytometry and other applications\• HTS and HCS—EnSens® substrates are naturally resistant to degradation\• High Signal Stability, resistant to photobleaching over 48 hours\• Easy: Mix and Read. No antibodies; no special equipment or complicated data analysis\\Find out more at www.enziumlabs.com.
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